The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects
- PMID:17631127
- DOI:10.1053/j.gastro.2007.04.005
The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects
Abstract
Background & aims:Irritable bowel syndrome (IBS) is a significant gastrointestinal disorder with unknown etiology. The symptoms can greatly weaken patients' quality of life and account for notable economical costs for society. Contribution of the gastrointestinal microbiota in IBS has been suggested. Our objective was to characterize putative differences in gastrointestinal microbiota between patients with IBS and control subjects. These differences could potentially have a causal relationship with the syndrome.
Methods:Microbial genomes from fecal samples of 24 patients with IBS and 23 controls were collected, pooled in a groupwise manner, and fractionated according to their guanine cytosine content. Selected fractions were analyzed by extensive high-throughput 16S ribosomal RNA gene cloning and sequencing of 3753 clones. Some of the revealed phylogenetic differences were further confirmed by quantitative polymerase chain reaction assays on individual samples.
Results:The coverage of the clone libraries of IBS subtypes and control subjects differed significantly (P < .0253). The samples were also distinguishable by the Bayesian analysis of bacterial population structure. Moreover, significant (P < .05) differences between the clone libraries were found in several bacterial genera, which could be verified by quantitative polymerase chain reaction assays of phylotypes belonging to the genera Coprococcus, Collinsella, and Coprobacillus.
Conclusions:The study showed that fecal microbiota is significantly altered in IBS. Further studies on molecular mechanisms underlying these alterations are needed to elucidate the exact role of intestinal bacteria in IBS.
Comment in
-
Irritable bowel syndrome: shifting the focus toward the gut microbiota.Gastroenterology. 2007 Jul;133(1):340-2. doi: 10.1053/j.gastro.2007.05.030. Gastroenterology. 2007. PMID:17631152 No abstract available.
-
Studying the human microbiome: beyond the petri dish.Curr Gastroenterol Rep. 2008 Aug;10(4):393-4. Curr Gastroenterol Rep. 2008. PMID:18627651 No abstract available.
Similar articles
-
Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR.Am J Gastroenterol. 2005 Feb;100(2):373-82. doi: 10.1111/j.1572-0241.2005.40312.x. Am J Gastroenterol. 2005. PMID:15667495
-
Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers.BMC杂志。2009年12月17日,9:95。doi: 10.1186 /1471-230X-9-95. BMC Gastroenterol. 2009. PMID:20015409 Free PMC article.
-
Global and deep molecular analysis of microbiota signatures in fecal samples from patients with irritable bowel syndrome.Gastroenterology. 2011 Nov;141(5):1792-801. doi: 10.1053/j.gastro.2011.07.043. Epub 2011 Aug 5. Gastroenterology. 2011. PMID:21820992
-
Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification.World J Gastroenterol. 2009 Dec 21;15(47):5936-45. doi: 10.3748/wjg.15.5936. World J Gastroenterol. 2009. PMID:20014457 Free PMC article.
-
Gastrointestinal microbiota in irritable bowel syndrome: their role in its pathogenesis and treatment.Am J Gastroenterol. 2008 Jun;103(6):1557-67. doi: 10.1111/j.1572-0241.2008.01869.x. Epub 2008 May 29. Am J Gastroenterol. 2008. PMID:18513268 Review.
Cited by
-
Effects of OsomeFood Clean Label plant-based meals on the gut microbiome.BMC Microbiol. 2023 Mar 30;23(1):88. doi: 10.1186/s12866-023-02822-z. BMC Microbiol. 2023. PMID:36997838 Free PMC article.
-
Collinsella aerofaciensProduces a pH-Responsive Lipid Immunogen.J Am Chem Soc. 2023 Apr 5;145(13):7071-7074. doi: 10.1021/jacs.3c00250. Epub 2023 Mar 23. J Am Chem Soc. 2023. PMID:36952265 Free PMC article.
-
Procedures in Fecal Microbiota Transplantation for Treating Irritable Bowel Syndrome: Systematic Review and Meta-Analysis.J Clin Med. 2023 Feb 21;12(5):1725. doi: 10.3390/jcm12051725. J Clin Med. 2023. PMID:36902512 Free PMC article. Review.
-
Effect of High Efficiency Digestion and Utilization of Organic Iron Made bySaccharomyces cerevisiae在抗氧化作用和盲肠微生物群落在断奶Piglets.Animals (Basel). 2023 Jan 31;13(3):498. doi: 10.3390/ani13030498. Animals (Basel). 2023. PMID:36766387 Free PMC article.
-
The role of the gut microbiome in paediatric irritable bowel syndrome.AIMS Microbiol. 2022 Nov 22;8(4):454-469. doi: 10.3934/microbiol.2022030. eCollection 2022. AIMS Microbiol. 2022. PMID:36694592 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases