摘要

虽然原癌基因的激活与许多组织癌症的发展和进展有关，但癌基因在食管腺癌发展中的作用尚未确定。研究了15例食管腺癌切除术患者和15例食管切除术或Barrett食管活检患者。后者患者也对邻近的正常胃粘膜进行活检，以与化生的食管粘膜进行比较。将粘膜样本快速冷冻，随后用以下癌基因相关蛋白的单克隆抗体染色;c-erbB2 (neu和CE-1)(外部结构域)，c-erbB2 (NCL-CB11)(内部结构域)，c-src, c-ras, c-myc, c-fos, c-jun，以及抑癌基因p53。所有肿瘤均为发生于食管下三分之一的分化良好或中等程度的腺癌。11个标本显示c-erbB2 (neu)和c-erbB2 (CBL-CB11)均有强烈的膜性染色。7例标本显示p53抑癌基因强核染色。3例c-ras和c-src阳性，2例c-jun阳性。在Barrett上皮中，9例c-erbB2阳性(neu和CB11)， 3例c-src阳性，2例c-ras和c-jun阳性，1例c-fos阳性。 Two of the gastric mucosal biopsy specimens expressed c-erbB2 weakly but no other oncogenes were found. The frequency of positive staining for c-erbB2 is very high, compared with the expression of these genes in other tumours. It is also concluded that errors in the onco-suppressor gene p53, and especially in the external and internal domains of c-erbB2, which is also often expressed in Barrett's mucosa, may be implicated in the development of adenocarcinoma of the oesophagus.