文摘

细胞核周围的antineutrophil胞质抗体最近被证明在炎症性肠病的患者的血清。从120例溃疡性结肠炎患者血清三百六十六份,105名患者患有克罗恩氏病和49非炎症肠道疾病控制在两个实验室测试,使用间接免疫荧光法测定。此外,fixed-neutrophil酶联immunoadsorbent测定(ELISA)在两个实验室的评估。免疫荧光试验中的结果显示两个实验室之间的高度相关性(Kappa系数= 0.8)。九十五年的120(79%)溃疡性结肠炎患者积极的测试只有105(13%)14节段性回肠炎患者积极的。血清从四个病人患有原发性硬化性胆管炎45的积极以及四个控制血清(9%)。细胞核周围的的敏感性antineutrophil胞质抗体免疫荧光试验对溃疡性结肠炎的诊断为0.75与0.88的特异性和阳性预测值为0.88(血清)。94年的ELISA技术37溃疡性结肠炎血清和68克罗恩病血清呈阳性。在对照组中只有一个病人患有原发性硬化性胆管炎反应积极(32个非炎症肠病血清测试)。ELISA技术有较高的特异性(0.97),但低敏感性(0.39)。 There was no relation of perinuclear antineutrophil cytoplasmic antibodies in ulcerative colitis patients or in Crohn's disease patients with disease activity, duration of illness, localisation, extent of disease, previous bowel operations or medical treatment. The clinical significance of perinuclear antineutrophil cytoplasmic antibody positive and negative subsets in both groups of patients thus remains unexplained. Our study confirms that determination of serum antineutrophil cytoplasmatic antibodies in patients with inflammatory bowel disease may differentiate ulcerative colitis from Crohn's disease. Further immunological studies are needed to explain the absence of these antibodies in a subset of ulcerative colitis patients and their role in the pathogenesis of the disease.