文摘

自身免疫已经与溃疡性结肠炎(UC)的发病机理。几项研究已经显示放大免疫球蛋白G1 (IgG1)抗体反应在加州大学;然而,免疫反应性的抗原(s)是未知的。研究这种抗原(s),粘膜结肠提取是由声波降解法,超速离心法紧随其后的是离子交换色谱法快速蛋白液相色谱。分数(丰富结肠肽),最新颖的单克隆抗体活性,7 e12h12 (IgM同形像),是孤立的,用来检查对病人的血清样本的免疫反应性。二百一十三编码样本111 UC患者(症状和治疗(63),症状和治疗(26),缓解(22));47与克罗恩病(CD)(30 40症状和治疗,结肠疾病);29急性腹泻引起的特定病原体(s);10与系统性红斑狼疮,16日正常受试者对丰富结肠检查肽通过免疫球蛋白亚型特定的酶联免疫吸附测定(elisa)。总IgG抗体反应显著(p < 0.01)高于只有在症状和治疗UC患者每个non-UC组相比,但灵敏度只有50%。 IgG2 and IgG3 reactivities were not different among various groups. The IgG1 antibody reactivity against the enriched colonic peptide, however, differentiated UC patients from CD and each of the other non-UC groups. Seventy nine per cent of the patients with UC, treated or untreated, symptomatic or in remission, had significantly (p < 0.0001) higher IgG1 antibody against the enriched colonic peptide when compared with each of the other non-UC groups. Only 12% of CD serum samples and none of the other control serum samples reacted. Using purified serum IgG1 and 7E12H12-IgM, by 7E12H12 reactive peptide indeed reacts with UC-IgG1 antibody but not with control IgG1.