摘要

隐窝脓肿可使活化的中性粒细胞长时间附着在结肠上皮表面。中性粒细胞与血管内皮和基底外侧上皮膜的粘附具有共同的效应分子，但过程不同。本研究旨在确定影响根尖上皮粘附的机制，而不依赖于移植。HT29 (cl 19A)细胞在以下条件下与中性粒细胞孵育并融合:(i)用豆蔻酸磷刺激中性粒细胞;(ii)用干扰素γ、肿瘤坏死因子α (IFN γ、TNF α)进行单层刺激;(iii)近期上皮细胞胰蛋白酶化。这些实验是在CD18、CD11b、LFA-1、e -选择素、p -选择素、细胞内粘附分子1 (ICAM-1)和ICAM-2中和抗体的存在下进行的;一种新型CD11b/CD18拮抗剂，中性粒细胞抑制因子(rNIF);腺苷受体激动剂(5' n -乙基羧胺腺苷/ n6 -环戊基腺苷(NECA/CPA))和血小板激活因子(PAF)受体拮抗剂lexipafant。受刺激中性粒细胞与静息单层的粘附依赖于Mac-1、CD18，独立于ICAM-1、ICAM-2、e -选择素、p -选择素和PAF。 Cytokine activated monolayers exhibited higher binding of neutrophils which was inhibited by rNIF and aCD18. Recently trypsinised monolayers bound neutrophils in a CD11b/CD18 and CD18 independent manner. Adenosine agonists failed to influence neutrophil adhesion under any condition. This study shows neutrophil adhesion to apical epithelial membranes is similar to that at the epithelial basolateral membrane, though different to that seen at the vascular endothelium. These results highlight regional differences in neutrophil adhesion molecule usage.