条文本
摘要
背景:l -精氨酸已被证明可诱导人空肠液体分泌。一氧化氮是l -精氨酸的衍生物,被认为是肠道促分泌剂的重要角色。目的:探讨l -精氨酸和一氧化氮合酶抑制剂硝基l -精氨酸甲酯(L-NAME)对大鼠空肠液电解质运动的影响。方法:用含(1)生理盐水,(2)d -精氨酸20,(3)l -精氨酸20,(4)l - name 0.1, 1,或20 mmol/l,或(5)l -精氨酸20和l - name 0.1, 1,或20 mmol/l的等渗透溶液原位灌注25 cm大鼠空肠段。在进一步的组中,皮下注射L-NAME 100 mg/kg对预处理d -或l -精氨酸500 mg/kg的大鼠的影响进行了检查。结果:l -精氨酸与d -精氨酸不同,尽管吸收更好(平均-7.3 v 17.0微升/分钟/克;P < 0.01)。0.1 mmol/l的l - name对基础液体运动没有影响,但逆转了l -精氨酸诱导的分泌(7.8;P < 0.05)。l - name在1和20 mmol/l时诱导液体分泌(分别为-15.4和-28.4),添加l -精氨酸(分别为-30.0和-41.0)可增强液体分泌; both p < 0.05). A subcutaneous injection of L-NAME resulted in marked fluid secretion (-39.9) and histological evidence of intestinal ischaemia. These changes were attenuated or reversed by pretreatment with subcutaneous L- but not D-arginine. CONCLUSIONS: L-arginine induces intestinal fluid secretion through production of nitric oxide. There is a delicate balance between the effect of nitric oxide as a secretagogue and its effect on maintaining blood flow and thus preventing intestinal ischaemia.