文摘

背景:革兰氏阴性脓毒性事件是发病率和死亡率的最常见的来源,由于手术病人有偏见的。大肠提供了哺乳动物和革兰氏阴性细菌的主要来源是涉及系统性endotoxaemia阻塞性黄疸的发病机制。胆汁盐有一个重要的部分在维护土著菌种内稳态通过乳化性质。目的:目的是探讨胆道梗阻和孤立的影响外部胆道分流肠道结构和盲肠的菌群与细菌易位。方法:成年雄性Wistar鼠六组进行了研究(没有操作,虚假的操作,和胆管结扎(BDL)和三个星期,一个choledocho-vesical瘘(CDVF)一周)。在研究结束时,等离子体是化验的证据endotoxaemia和动物肠道细菌易位检测肠系膜淋巴结复杂(MLNC)、肝、肺、脾。进行了定量和定性的细菌学研究盲肠的内容和段结肠和回肠末端清洗和准备组织学评估。结果:细菌易位是BDL1显著增加(68.8%)和BDL3(60%)组与sham1相比(6.3%),sham3(9.1%),没有操作(0%),CDVF1(16.7%)组。虽然易位是BDL1组更为明显,这是几乎完全MLNC相比更广泛易位BDL3组的其他器官。BDL3组是唯一一组内毒素浓度显著提高和anticore醣脂类。 The caecal Gram negative aerobic counts were significantly increased in the BDL1 and CDVF1 groups compared with all other groups. There was evidence of structural abnormalities in the terminal ileum of rats jaundiced for three weeks, but not in the other groups. CONCLUSIONS: Biliary obstruction for one and three weeks promotes bacterial translocation although the mechanisms may be different. Absence of intralumenal bile results in a significant but self limiting increase in the Gram negative aerobic population, which may account for translocation in the early stages of biliary obstruction. As the duration of biliary obstruction increases systemic endotoxaemia is a consistent feature which, combined with factors such as immunological depression and physical disruption of gut barrier function, may promote bacterial translocation perpetuating systemic sepsis.